Somaclonal Variation of Three Tolerance Streptomycetes Isolated from Saline Soil

The present investigation aims to the isolation of Streptomycetes from saline soil. The isolates were varied in color to aerial vegetative mycelia, morphology of conidospore surface, melanin production, utilization of carbon; nitrogen source and fatty acid analysis, as well as physical and chemical characters for their habitat soil. Streptomycetes isolates exhibiting wide range of variability. Analysis of variance for their characters revealed the presence of genotypic differences. The tolerance of sodium chloride indicates that almost the characters were less influenced by the environment. DNA finger print analysis were successfully revealed somaclonal and DNA genetic divesity among three isolates closely related St. catvus; St.canaries and St., nogalater. Randomly amplified polymorphic DNA (RAPD) for identification of three Streptomyces isolates were conducted on the screening of the primers and on the extraction of template DNA. Three out of ten random sequence 10 mer primers were successful in the three isolates ST1, ST2 and ST3 resemble to genus Streptomyces. PCR amplification yielded reproducible PAPD fragments which differentiated the three isolates examined. The difference was also recognized among the RAPD Fragments especially of St. calvus. RAPD-PCR analysis can be used to gain rapid and precise information about genetic similarities and dissimilarities of three Streptomycetes isolates. [Seham M. Shash. Somaclonal Variation of Three Tolerance Streptomycetes Isolated from Saline Soil. New York Science Journal 2011;4(12):67-73]. (ISSN: 1554-0200).